Densitometric evaluation of spiraeoside after derivatization in flowers of Filipendula ulmaria (L.) Maxim.

In traditional medicines of Europe, thé water extract of Filipendula ulmaria (L.) Maxim. (Rosaceae) flowers has been used as antiinflammatory, analgésie and diuretic (1) and thé compounds of this plant are known to be flavonoids tannins and salicylic acid derivatives (2). Spiraeoside (quercetin-4 glucoside) is thé major and characteristic flavonoid of Filipendula ulmaria flowers and, for this reason, we determined its amount by HPTLC densitometry. We measured thé fluorescence of spiraeoside after derivatization by diphenylboric acid-2-aminoethylester (3). The measurement was achieved by means of a TLC Scanner programmed to work in reflection fluorescence at 330 nm (Mercury lamp, cut-off filter 450 nm). We respected thé following chromatographic procédure: Layer: HPTLC plates silicagel 60 Merck Mobile phase: ethylacetate-formic acid-water (6:1:1 ,v/v/v) Standard solution (0.6,0.8 and 1 ni). 4 mg of spiraeoside SCR in 10 ml methanol. Sample solution: (0.4 M.!): 0.250 g of Filipendula flowers were extracted by 25 ml methanol 60°C (2 hours). The solution was fittrated, evaporated and dissolved in 25 ml methanol. After linearization, thé concentration of spiraeoside was estimated by measurement of thé différent standard and samples mean areas. In ourfindings, thé spiraeoside content ranged from 3 to 4.3 %. The repeatability, reproducibility and thé good linearity were confirmed by thé validation of thé method. This HPTLC method is rapid and suitable for adoption in thé future for thé détermination of spiraeoside in Filipendula ulmaria flowers and extracts. Références 1) Yeo, H.S., Kim, J., Chung, B.S. (1990) Planta Med. 56, 539. 2) Meier, B., Lehmann, D., Sticher, O, Bettschart, A. (1987) Dtsch Apoth. Ztg, 127, 2401-2407. 3) Billeter, M., Meier, B., Sticher, O. (1990) J. Planar Chromatogr. 3,370-375. h I ,